Variability of the human immunodeficiency virus type 1 polymerase gene from treatment naive patients in Accra, Ghana
Identifieur interne : 000D34 ( Main/Exploration ); précédent : 000D33; suivant : 000D35Variability of the human immunodeficiency virus type 1 polymerase gene from treatment naive patients in Accra, Ghana
Auteurs : Kwamena William Coleman Sagoe [Ghana] ; Magdar Dwidar [États-Unis] ; Margaret Lartey [Ghana] ; Isaac Boamah [Ghana] ; AFRAKOMA ADJOA AGYEI [Ghana] ; ANNA ABA HAYFORD [Ghana] ; JULIUS ABRAHAM ADDO MINGLE [Ghana] ; Max Q. Arens [États-Unis]Source :
- Journal of clinical virology [ 1386-6532 ] ; 2007.
Descripteurs français
- Pascal (Inist)
- Wicri :
English descriptors
- KwdEn :
- AIDS, Gene, Ghana, HIV-1 virus, Human, Microbiology, Mutation, Resistance, Subtype, Treatment, Virology.
Abstract
Background: Little is known about the HIV-1 drug resistance mutations in Ghana. Objectives: To determine the background protease (PR) and reverse transcriptase (RT) mutations of HIV-1 from treatment naive patients in Ghana. Study design: Twenty-five plasma samples randomly selected were analyzed for drug resistance mutations. The molecular phylogeny and recombinant patterns of the polymerase gene of HIV-1 were also analysed. Results: No major drug-resistance mutations were seen in protease or reverse transcriptase genes. The L10I, L10V, V11I and E35G minor mutations were seen in four patients, while the V179E was observed in a patient with subtype G. An insertion of lysine was found at codon 36 of the protease gene of one patient. The predominant subtype was the CRF02_AG strain (n = 22), but 3 (13.6%) of these were recombinants with HIV- 1 subtype K and/or A 1. Two patients harboured unclassified/complex strains with D/CRF01 -AE and G/CRFAG_02 subtypes for the PR and RT, respectively, using the Stanford Database. Viral loads (VL) ranged from 2290 to >1,500,000 c/ml (mean = 339,065 c/ml). Conclusions: Treatment naive patients in Ghana before scale-up may have minor but not major PR mutations and high viral loads. The clinical effects of minor mutations/polymorphisms in the PR and RT genes and recombinants need to be investigated.
Affiliations:
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Box 4236</s1><s2>Accra</s2><s3>GHA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Dwidar, Magdar" sort="Dwidar, Magdar" uniqKey="Dwidar M" first="Magdar" last="Dwidar">Magdar Dwidar</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Retrovirus Laboratory, Department of Pediatrics, Washington University Medical School</s1><s2>St. Louis, MO 63110</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Missouri (État)</region></placeName></affiliation></author><author><name sortKey="Lartey, Margaret" sort="Lartey, Margaret" uniqKey="Lartey M" first="Margaret" last="Lartey">Margaret Lartey</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Department of Medicine, University of Ghana Medical School, P.O. 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Box 4236</s1><s2>Accra</s2><s3>GHA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Julius Abraham Addo Mingle" sort="Julius Abraham Addo Mingle" uniqKey="Julius Abraham Addo Mingle" last="Julius Abraham Addo Mingle">JULIUS ABRAHAM ADDO MINGLE</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Clinical Virology Laboratory (Department of Microbiology), University of Ghana Medical School, P.O. Box 4236</s1><s2>Accra</s2><s3>GHA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Arens, Max Q" sort="Arens, Max Q" uniqKey="Arens M" first="Max Q." last="Arens">Max Q. Arens</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Retrovirus Laboratory, Department of Pediatrics, Washington University Medical School</s1><s2>St. Louis, MO 63110</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Missouri (État)</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">07-0480572</idno><date when="2007">2007</date><idno type="stanalyst">PASCAL 07-0480572 INIST</idno><idno type="RBID">Pascal:07-0480572</idno><idno type="wicri:Area/PascalFrancis/Corpus">000083</idno><idno type="wicri:Area/PascalFrancis/Curation">000059</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000066</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000066</idno><idno type="wicri:doubleKey">1386-6532:2007:Coleman Sagoe K:variability:of:the</idno><idno type="wicri:Area/Main/Merge">000D66</idno><idno type="wicri:Area/Main/Curation">000D34</idno><idno type="wicri:Area/Main/Exploration">000D34</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Variability of the human immunodeficiency virus type 1 polymerase gene from treatment naive patients in Accra, Ghana</title><author><name sortKey="Coleman Sagoe, Kwamena William" sort="Coleman Sagoe, Kwamena William" uniqKey="Coleman Sagoe K" first="Kwamena William" last="Coleman Sagoe">Kwamena William Coleman Sagoe</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Clinical Virology Laboratory (Department of Microbiology), University of Ghana Medical School, P.O. Box 4236</s1><s2>Accra</s2><s3>GHA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Dwidar, Magdar" sort="Dwidar, Magdar" uniqKey="Dwidar M" first="Magdar" last="Dwidar">Magdar Dwidar</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Retrovirus Laboratory, Department of Pediatrics, Washington University Medical School</s1><s2>St. Louis, MO 63110</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Missouri (État)</region></placeName></affiliation></author><author><name sortKey="Lartey, Margaret" sort="Lartey, Margaret" uniqKey="Lartey M" first="Margaret" last="Lartey">Margaret Lartey</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Department of Medicine, University of Ghana Medical School, P.O. Box 4236</s1><s2>Accra</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName><orgName type="university" n="3">Université du Ghana</orgName></affiliation></author><author><name sortKey="Boamah, Isaac" sort="Boamah, Isaac" uniqKey="Boamah I" first="Isaac" last="Boamah">Isaac Boamah</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Clinical Virology Laboratory (Department of Microbiology), University of Ghana Medical School, P.O. Box 4236</s1><s2>Accra</s2><s3>GHA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Afrakoma Adjoa Agyei" sort="Afrakoma Adjoa Agyei" uniqKey="Afrakoma Adjoa Agyei" last="Afrakoma Adjoa Agyei">AFRAKOMA ADJOA AGYEI</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Department of Medicine, University of Ghana Medical School, P.O. Box 4236</s1><s2>Accra</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Anna Aba Hayford" sort="Anna Aba Hayford" uniqKey="Anna Aba Hayford" last="Anna Aba Hayford">ANNA ABA HAYFORD</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Clinical Virology Laboratory (Department of Microbiology), University of Ghana Medical School, P.O. Box 4236</s1><s2>Accra</s2><s3>GHA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Julius Abraham Addo Mingle" sort="Julius Abraham Addo Mingle" uniqKey="Julius Abraham Addo Mingle" last="Julius Abraham Addo Mingle">JULIUS ABRAHAM ADDO MINGLE</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Clinical Virology Laboratory (Department of Microbiology), University of Ghana Medical School, P.O. Box 4236</s1><s2>Accra</s2><s3>GHA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Arens, Max Q" sort="Arens, Max Q" uniqKey="Arens M" first="Max Q." last="Arens">Max Q. Arens</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Retrovirus Laboratory, Department of Pediatrics, Washington University Medical School</s1><s2>St. Louis, MO 63110</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Missouri (État)</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of clinical virology</title><title level="j" type="abbreviated">J. clin. virol.</title><idno type="ISSN">1386-6532</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of clinical virology</title><title level="j" type="abbreviated">J. clin. virol.</title><idno type="ISSN">1386-6532</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>AIDS</term><term>Gene</term><term>Ghana</term><term>HIV-1 virus</term><term>Human</term><term>Microbiology</term><term>Mutation</term><term>Resistance</term><term>Subtype</term><term>Treatment</term><term>Virology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus HIV1</term><term>Homme</term><term>Gène</term><term>Traitement</term><term>Ghana</term><term>Soustype</term><term>Résistance</term><term>Mutation</term><term>Microbiologie</term><term>Virologie</term><term>SIDA</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Ghana</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: Little is known about the HIV-1 drug resistance mutations in Ghana. Objectives: To determine the background protease (PR) and reverse transcriptase (RT) mutations of HIV-1 from treatment naive patients in Ghana. Study design: Twenty-five plasma samples randomly selected were analyzed for drug resistance mutations. The molecular phylogeny and recombinant patterns of the polymerase gene of HIV-1 were also analysed. Results: No major drug-resistance mutations were seen in protease or reverse transcriptase genes. The L10I, L10V, V11I and E35G minor mutations were seen in four patients, while the V179E was observed in a patient with subtype G. An insertion of lysine was found at codon 36 of the protease gene of one patient. The predominant subtype was the CRF02_AG strain (n = 22), but 3 (13.6%) of these were recombinants with HIV- 1 subtype K and/or A 1. Two patients harboured unclassified/complex strains with D/CRF01 -AE and G/CRFAG_02 subtypes for the PR and RT, respectively, using the Stanford Database. Viral loads (VL) ranged from 2290 to >1,500,000 c/ml (mean = 339,065 c/ml). Conclusions: Treatment naive patients in Ghana before scale-up may have minor but not major PR mutations and high viral loads. The clinical effects of minor mutations/polymorphisms in the PR and RT genes and recombinants need to be investigated.</div></front></TEI><affiliations><list><country><li>Ghana</li><li>États-Unis</li></country><region><li>Missouri (État)</li><li>Région du Grand Accra</li></region><settlement><li>Accra</li><li>Legon (Ghana)</li></settlement><orgName><li>Université du Ghana</li></orgName></list><tree><country name="Ghana"><region name="Région du Grand Accra"><name sortKey="Coleman Sagoe, Kwamena William" sort="Coleman Sagoe, Kwamena William" uniqKey="Coleman Sagoe K" first="Kwamena William" last="Coleman Sagoe">Kwamena William Coleman Sagoe</name></region><name sortKey="Afrakoma Adjoa Agyei" sort="Afrakoma Adjoa Agyei" uniqKey="Afrakoma Adjoa Agyei" last="Afrakoma Adjoa Agyei">AFRAKOMA ADJOA AGYEI</name><name sortKey="Anna Aba Hayford" sort="Anna Aba Hayford" uniqKey="Anna Aba Hayford" last="Anna Aba Hayford">ANNA ABA HAYFORD</name><name sortKey="Boamah, Isaac" sort="Boamah, Isaac" uniqKey="Boamah I" first="Isaac" last="Boamah">Isaac Boamah</name><name sortKey="Julius Abraham Addo Mingle" sort="Julius Abraham Addo Mingle" uniqKey="Julius Abraham Addo Mingle" last="Julius Abraham Addo Mingle">JULIUS ABRAHAM ADDO MINGLE</name><name sortKey="Lartey, Margaret" sort="Lartey, Margaret" uniqKey="Lartey M" first="Margaret" last="Lartey">Margaret Lartey</name></country><country name="États-Unis"><region name="Missouri (État)"><name sortKey="Dwidar, Magdar" sort="Dwidar, Magdar" uniqKey="Dwidar M" first="Magdar" last="Dwidar">Magdar Dwidar</name></region><name sortKey="Arens, Max Q" sort="Arens, Max Q" uniqKey="Arens M" first="Max Q." last="Arens">Max Q. 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